Experimental and numerical study of the poplar plastocyanin isoforms using Tyr as a probe for electrostatic similarity and dissimilarity.
Identifieur interne : 004273 ( Main/Exploration ); précédent : 004272; suivant : 004274Experimental and numerical study of the poplar plastocyanin isoforms using Tyr as a probe for electrostatic similarity and dissimilarity.
Auteurs : A. Shosheva [Bulgarie] ; A. Donchev ; M. Dimitrov ; I. Zlatanov ; G. Toromanov ; V. Getov ; E. AlexovSource :
- Biochimica et biophysica acta [ 0006-3002 ] ; 2004.
Descripteurs français
- KwdFr :
- Acrylamide (métabolisme), Cinétique (MeSH), Concentration en ions d'hydrogène (MeSH), Fluorescence (MeSH), Isoformes de protéines (composition chimique), Isoformes de protéines (métabolisme), Plastocyanine (composition chimique), Plastocyanine (métabolisme), Populus (composition chimique), Populus (métabolisme), Sondes moléculaires (MeSH), Spectrophotométrie (MeSH), Structure tertiaire des protéines (MeSH), Tyrosine (MeSH), Électricité statique (MeSH).
- MESH :
- composition chimique : Isoformes de protéines, Plastocyanine, Populus.
- métabolisme : Acrylamide, Isoformes de protéines, Plastocyanine, Populus.
- Cinétique, Concentration en ions d'hydrogène, Fluorescence, Sondes moléculaires, Spectrophotométrie, Structure tertiaire des protéines, Tyrosine, Électricité statique.
English descriptors
- KwdEn :
- Acrylamide (metabolism), Fluorescence (MeSH), Hydrogen-Ion Concentration (MeSH), Kinetics (MeSH), Molecular Probes (MeSH), Plastocyanin (chemistry), Plastocyanin (metabolism), Populus (chemistry), Populus (metabolism), Protein Isoforms (chemistry), Protein Isoforms (metabolism), Protein Structure, Tertiary (MeSH), Spectrophotometry (MeSH), Static Electricity (MeSH), Tyrosine (MeSH).
- MESH :
- chemical , chemistry : Plastocyanin, Protein Isoforms.
- chemical , metabolism : Acrylamide, Plastocyanin, Protein Isoforms.
- chemistry : Populus.
- metabolism : Populus.
- Fluorescence, Hydrogen-Ion Concentration, Kinetics, Molecular Probes, Protein Structure, Tertiary, Spectrophotometry, Static Electricity, Tyrosine.
Abstract
A detailed study of the tyrosine spectral characteristics was carried out in a broad range of pHs for both isoforms of plastocyanin from poplar. It was found that Tyr 80 is always protonated while Tyr 83 can form a tirosinate at high pHs. The pK(a) of Tyr 83 is practically identical in plastocyanin a and b, but the quenching of its spectrum is different in the isoforms. This provides insights that the acidic patches surrounding Tyr 83 have different electrostatic properties in plastocyanin a and b. The protonation states and the electrostatic interactions were numerically modeled on the existing plastocyanin a structure and on a homology model of plastocyanin b. The results of numerical calculations agree with the experimental findings and identify several differences in the titration behavior of the acidic patches. The difference of the tyrosine quenching pH profiles of the isoforms is rationalized by the differences in the calculated pK(a)'s of amino acids in the neighboring acidic clusters.
DOI: 10.1016/j.bbapap.2003.10.010
PubMed: 15063316
Affiliations:
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Dimitrov</name></author><author><name sortKey="Zlatanov, I" sort="Zlatanov, I" uniqKey="Zlatanov I" first="I" last="Zlatanov">I. Zlatanov</name></author><author><name sortKey="Toromanov, G" sort="Toromanov, G" uniqKey="Toromanov G" first="G" last="Toromanov">G. Toromanov</name></author><author><name sortKey="Getov, V" sort="Getov, V" uniqKey="Getov V" first="V" last="Getov">V. Getov</name></author><author><name sortKey="Alexov, E" sort="Alexov, E" uniqKey="Alexov E" first="E" last="Alexov">E. 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Shosheva</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biophysics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.</nlm:affiliation><country xml:lang="fr">Bulgarie</country><wicri:regionArea>Institute of Biophysics, Bulgarian Academy of Sciences, Sofia 1113</wicri:regionArea><placeName><settlement type="city">Sofia</settlement><region nuts="2">Sofia-ville (oblast)</region></placeName></affiliation></author><author><name sortKey="Donchev, A" sort="Donchev, A" uniqKey="Donchev A" first="A" last="Donchev">A. Donchev</name></author><author><name sortKey="Dimitrov, M" sort="Dimitrov, M" uniqKey="Dimitrov M" first="M" last="Dimitrov">M. Dimitrov</name></author><author><name sortKey="Zlatanov, I" sort="Zlatanov, I" uniqKey="Zlatanov I" first="I" last="Zlatanov">I. Zlatanov</name></author><author><name sortKey="Toromanov, G" sort="Toromanov, G" uniqKey="Toromanov G" first="G" last="Toromanov">G. Toromanov</name></author><author><name sortKey="Getov, V" sort="Getov, V" uniqKey="Getov V" first="V" last="Getov">V. Getov</name></author><author><name sortKey="Alexov, E" sort="Alexov, E" uniqKey="Alexov E" first="E" last="Alexov">E. Alexov</name></author></analytic><series><title level="j">Biochimica et biophysica acta</title><idno type="ISSN">0006-3002</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrylamide (metabolism)</term><term>Fluorescence (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Kinetics (MeSH)</term><term>Molecular Probes (MeSH)</term><term>Plastocyanin (chemistry)</term><term>Plastocyanin (metabolism)</term><term>Populus (chemistry)</term><term>Populus (metabolism)</term><term>Protein Isoforms (chemistry)</term><term>Protein Isoforms (metabolism)</term><term>Protein Structure, Tertiary (MeSH)</term><term>Spectrophotometry (MeSH)</term><term>Static Electricity (MeSH)</term><term>Tyrosine (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acrylamide (métabolisme)</term><term>Cinétique (MeSH)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Fluorescence (MeSH)</term><term>Isoformes de protéines (composition chimique)</term><term>Isoformes de protéines (métabolisme)</term><term>Plastocyanine (composition chimique)</term><term>Plastocyanine (métabolisme)</term><term>Populus (composition chimique)</term><term>Populus (métabolisme)</term><term>Sondes moléculaires (MeSH)</term><term>Spectrophotométrie (MeSH)</term><term>Structure tertiaire des protéines (MeSH)</term><term>Tyrosine (MeSH)</term><term>Électricité statique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plastocyanin</term><term>Protein Isoforms</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acrylamide</term><term>Plastocyanin</term><term>Protein Isoforms</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Isoformes de protéines</term><term>Plastocyanine</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acrylamide</term><term>Isoformes de protéines</term><term>Plastocyanine</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fluorescence</term><term>Hydrogen-Ion Concentration</term><term>Kinetics</term><term>Molecular Probes</term><term>Protein Structure, Tertiary</term><term>Spectrophotometry</term><term>Static Electricity</term><term>Tyrosine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Concentration en ions d'hydrogène</term><term>Fluorescence</term><term>Sondes moléculaires</term><term>Spectrophotométrie</term><term>Structure tertiaire des protéines</term><term>Tyrosine</term><term>Électricité statique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A detailed study of the tyrosine spectral characteristics was carried out in a broad range of pHs for both isoforms of plastocyanin from poplar. It was found that Tyr 80 is always protonated while Tyr 83 can form a tirosinate at high pHs. The pK(a) of Tyr 83 is practically identical in plastocyanin a and b, but the quenching of its spectrum is different in the isoforms. This provides insights that the acidic patches surrounding Tyr 83 have different electrostatic properties in plastocyanin a and b. The protonation states and the electrostatic interactions were numerically modeled on the existing plastocyanin a structure and on a homology model of plastocyanin b. The results of numerical calculations agree with the experimental findings and identify several differences in the titration behavior of the acidic patches. The difference of the tyrosine quenching pH profiles of the isoforms is rationalized by the differences in the calculated pK(a)'s of amino acids in the neighboring acidic clusters.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15063316</PMID><DateCompleted><Year>2004</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>26</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-3002</ISSN><JournalIssue CitedMedium="Print"><Volume>1698</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Apr</Month><Day>08</Day></PubDate></JournalIssue><Title>Biochimica et biophysica acta</Title><ISOAbbreviation>Biochim Biophys Acta</ISOAbbreviation></Journal><ArticleTitle>Experimental and numerical study of the poplar plastocyanin isoforms using Tyr as a probe for electrostatic similarity and dissimilarity.</ArticleTitle><Pagination><MedlinePgn>67-75</MedlinePgn></Pagination><Abstract><AbstractText>A detailed study of the tyrosine spectral characteristics was carried out in a broad range of pHs for both isoforms of plastocyanin from poplar. It was found that Tyr 80 is always protonated while Tyr 83 can form a tirosinate at high pHs. The pK(a) of Tyr 83 is practically identical in plastocyanin a and b, but the quenching of its spectrum is different in the isoforms. This provides insights that the acidic patches surrounding Tyr 83 have different electrostatic properties in plastocyanin a and b. The protonation states and the electrostatic interactions were numerically modeled on the existing plastocyanin a structure and on a homology model of plastocyanin b. The results of numerical calculations agree with the experimental findings and identify several differences in the titration behavior of the acidic patches. The difference of the tyrosine quenching pH profiles of the isoforms is rationalized by the differences in the calculated pK(a)'s of amino acids in the neighboring acidic clusters.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shosheva</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Biophysics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Donchev</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Dimitrov</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Zlatanov</LastName><ForeName>I</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Toromanov</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Getov</LastName><ForeName>V</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Alexov</LastName><ForeName>E</ForeName><Initials>E</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biochim Biophys Acta</MedlineTA><NlmUniqueID>0217513</NlmUniqueID><ISSNLinking>0006-3002</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015335">Molecular Probes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020033">Protein Isoforms</NameOfSubstance></Chemical><Chemical><RegistryNumber>20R035KLCI</RegistryNumber><NameOfSubstance UI="D020106">Acrylamide</NameOfSubstance></Chemical><Chemical><RegistryNumber>42HK56048U</RegistryNumber><NameOfSubstance UI="D014443">Tyrosine</NameOfSubstance></Chemical><Chemical><RegistryNumber>9014-09-9</RegistryNumber><NameOfSubstance UI="D010970">Plastocyanin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020106" MajorTopicYN="N">Acrylamide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005453" MajorTopicYN="N">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015335" MajorTopicYN="N">Molecular Probes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010970" MajorTopicYN="N">Plastocyanin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020033" MajorTopicYN="N">Protein Isoforms</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013053" MajorTopicYN="N">Spectrophotometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055672" MajorTopicYN="N">Static Electricity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014443" MajorTopicYN="N">Tyrosine</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2003</Year><Month>07</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2003</Year><Month>10</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2003</Year><Month>10</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>4</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>5</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>4</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15063316</ArticleId><ArticleId IdType="doi">10.1016/j.bbapap.2003.10.010</ArticleId><ArticleId IdType="pii">S1570963903003212</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Bulgarie</li></country><region><li>Sofia-ville (oblast)</li></region><settlement><li>Sofia</li></settlement></list><tree><noCountry><name sortKey="Alexov, E" sort="Alexov, E" uniqKey="Alexov E" first="E" last="Alexov">E. Alexov</name><name sortKey="Dimitrov, M" sort="Dimitrov, M" uniqKey="Dimitrov M" first="M" last="Dimitrov">M. Dimitrov</name><name sortKey="Donchev, A" sort="Donchev, A" uniqKey="Donchev A" first="A" last="Donchev">A. Donchev</name><name sortKey="Getov, V" sort="Getov, V" uniqKey="Getov V" first="V" last="Getov">V. Getov</name><name sortKey="Toromanov, G" sort="Toromanov, G" uniqKey="Toromanov G" first="G" last="Toromanov">G. Toromanov</name><name sortKey="Zlatanov, I" sort="Zlatanov, I" uniqKey="Zlatanov I" first="I" last="Zlatanov">I. Zlatanov</name></noCountry><country name="Bulgarie"><region name="Sofia-ville (oblast)"><name sortKey="Shosheva, A" sort="Shosheva, A" uniqKey="Shosheva A" first="A" last="Shosheva">A. Shosheva</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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